Compared to classical examples, biocompatible reversible fluorescent probes that respond reversibly to a redox signal are an emerging class of molecules especially for the detection of reactive oxygen and nitrogen species involved in oxidative stress. Accordingly, they play a crucial role to distinguish transient and chronic changes in oxidative capacity in living organisms. In this context, our goal is to develop original biocompatible and reversible redox-responsive fluorescent metallocene-based probes able to target cell membranes and/or subcellular organelles. Their design features the association of an organic fluorophore and a metallocene in order to promote a redox-switchable Photo-induced Electron Transfer (PeT) as the key-step of fluorescence control.