The axis focuses on the design, synthesis and characterization of peptides and/or proteins modulating biological interactions.

Chemical ligation methodologies are being developed for the synthesis of proteins incorporating non-canonical amino acids or post-translational modifications.

Cyclic peptides with enhanced pharmacological properties are conceived for the design of protein-protein interaction inhibitors (PPIs) in oncology and virology or as biocondensates modulators or glycosaminoglycan ligands for cell targeting. In parallel, approaches based on dynamic combinatorial chemistry (DCC) are developed for the screening and selection of constrained peptides. Large dynamic combinatorial libraries (DCL) of peptides are characterized by the means of separative techniques coupled with MS and tandem MS using various types of ions activation

++Associated methodologies:++

• Synthesis of modified amino acids, peptides and proteins on solid and in solution (F. Burlina, R. Moumné, L.
• Native chemical ligation and auxiliary-mediated ligation (F. Burlina)
• Functionalization of peptides by dynamic combinatorial chemistry (R. Moumné, L. Rocard)
• MS and MS/MS of polycationic, cyclic, gas-modified peptide ions (E. Sachon)
• Protein production and purification (D. Ravault)