Tissue repair and embryogenesis both involve variations in H2O2 levels. The team works on zebrafish, which is a transparent organism in which optical probes can be followed. Tracking H2O2 levels in zebrafish expressing a genetically encoded H2O2 probe (HyPer) is possible, for example at the scar of a severed fin or during embryogenesis.

We study the role of redox signaling during regeneration in adult zebrafish: the distribution of H2O2 shows an increasing concentration gradient towards the scar. H2O2 distribution also varies during morphogenesis, with waves in time and space.

Collaborators:

• S. Thenet & V. Carrière, CRSA, Paris

References: 21 C. Rampon et al., Antioxidants, 2018, 7, 159. 22 M. Thauvin et al., Journal of Cell Science, 2022, 135, jcs259664. 23 C. Gauron et al., Dev Biol, 2016, 414, 133–141.