Dissolution Dynamic Nuclear Polarization (dDNP) provides signal enhancement in solution Nuclear Magnetic Resonance (NMR) experiments by up to four orders of magnitude. Since its introduction a couple of decades ago, it has opened new prospects, in particular for the study of fast (< ~min) chemical reaction. On the one hand, i the field of biology, it allows the observation of chemical and enzymatic reactions on (fast) physiological time scales and using nuclei of lower sensitivity. There remains a number of obstacles (technical, instrumentation related) that need to be overcome and we develop dDNP methodology to advance the approach to the stage of an analytical technique. We apply dDNP to the study of several enzymes involved in the pentose phosphate pathway and in the metabolism of glutamine, both metabolic pathways of fundamental importance. On the other hand, we apply and develop dDNP methodology for the study of chemical reactions, such as the CO2 capture and release by amine solutions, of widespread use for the storage of CO2 in the industrial context.